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|Section2= |Section3= }} Rhodamine B is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. Rhodamine B is used in biology as a staining fluorescent dye, sometimes in combination with auramine O, as the auramine-rhodamine stain to demonstrate acid-fast organisms, notably ''Mycobacterium''. Rhodamine B is tunable around 610 nm when used as a laser dye.〔(Rhodamine B )〕 Its luminescence quantum yield is 0.65 in basic ethanol, 0.49 in ethanol, 1.0, and 0.68 in 94% ethanol. The fluorescence yield is temperature dependent. ==Solubility== The solubility of Rhodamine B in water is ~15 g/L.〔''Sicherheitsdatenblatt Rhodamin B'' by Roth, 2013〕 However, the solubility in acetic acid solution (30 vol.%) is ~400 g/L. Chlorinated tap water decomposes rhodamine B. Rhodamine B solutions are often absorbed in plastics therefore , it should be kept in glass.〔''Detection and prevention of leaks from dams'' By Antonio Plata Bedmar and Luís Araguás Araguás, Taylor & Francis, 2002, ISBN 90-5809-355-7〕 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Rhodamine B」の詳細全文を読む スポンサード リンク
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